Analytical Data
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基因名
F5
- Application
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别名
F5；HTR1EL；5-hydroxytryptamine receptor 1F
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P30939
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表达区间
1-366aa
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氨基酸序列
MDFLNSSDQNLTSEELLNRMPSKILVSLTLSGLALMTTTINSLVIAAIIVTRKLHHPANYLICSLAVTDFLVAVLVMPFSIVYIVRESWIMGQVVCDIWLSVDITCCTCSILHLSAIALDRYRAITDAVEYARKRTPKHAGIMITIVWIISVFISMPPLFWRHQGTSRDDECIIKHDHIVSTIYSTFGAFYIPLALILILYYKIYRAAKTLYHKRQASRIAKEEVNGQVLLESGEKSTKSVSTSYVLEKSLSDPSTDFDKIHSTVRSLRSEFKHEKSWRRQKISGTRERKAATTLGLILGAFVICWLPFFVKELVVNVCDKCKISEEMSNFLAWLGYLNSLINPLIYTIFNEDFKKAFQKLVRCRC
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分子量
41.7 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of F5 recombinant proteins is rooted in the critical importance of coagulation factors in maintaining hemostasis and their roles in various pathological conditions. F5, also known as factor V, is a crucial cofactor in the prothrombinase complex that catalyzes the conversion of prothrombin to thrombin, which is essential for blood clot formation. Dysregulation or mutations in the F5 gene can lead to clotting disorders such as thrombophilia or hemophilia, leading to serious health risks. The advent of recombinant DNA technology has enabled the production of F5 and its variants in controlled laboratory settings, facilitating detailed studies on their structure and function. This research aims to elucidate the molecular mechanisms governing coagulation, explore therapeutic applications, and provide insights into the development of inhibitors for anticoagulant therapy. Additionally, recombinant F5 proteins serve as valuable tools in diagnostic assays and as research reagents. The ongoing investigation into the biophysical properties, biological activity, and potential medical applications underscores the significance of F5 recombinant proteins in advancing our understanding of coagulation pathways and improving patient outcomes in clotting disorders.
