Analytical Data
-
基因名
IL-4RA
-
简介
IL-4R alpha is a subunit alpha shared by IL-4 and IL-13 receptors, found in leukocytes originally. IL-4R alpha couples to the JAK1/2/3-STAT6 pathway and involves in promoting Th2 differentiation[1]. IL-4R alpha/CD124, Human consists of 825 amino acids (M1-S825) with a transmembrane domain (233-256 a.a) and a soluble form (1-227 a.a). Soluble IL-4R (sIL-4R) inhibits IL4-mediated cell proliferation and IL-5 up-regulation by T-cells[1]. IL-4R alpha/CD124, Human (M26-Q231) is produced in HEK293 cells with a C-Terminal hFc-tag.
- Application
-
生物活性
1.The ability to inhibit IL-4-dependent proliferation of TF1 human erythroleukemic cells has an ED50 value of 5-20 ng/mL. 2.Immobilized Human IL-4 RA-Fc at 5 μg/mL (100 μl/well) can bind Human IL-4 *: Biotinylated by NHS-biotin prior to testing.The ED50 of Human IL-4 is 2.43 ng/mL. 3.Loaded Human IL-4-His on HIS1K Biosensor, can bind Human IL-4RA-Fc with an affinity constant of 0.12 nM as determined in BLI assay.
-
别名
Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD124; IL-4-binding protein; IL4-BP; IL4R; IL4RA
-
种属
Human
-
表达系统
HEK293
-
标签
C-hFc
-
纯度
> 90% as determined by SDS-PAGE.
-
蛋白编号
P24394
-
表达区间
M26-Q231
-
氨基酸序列
MKVLQEPTCVSDYMSISTCEWKMNGPTNCSTELRLLYQLVFLLSEAHTCIPENNGGAGCVCHLLMDDVVSADNYTLDLWAGQQLLWKGSFKPSEHVKPRAPGNLTVHTNVSDTLLLTWSNPYPPDNYLYNHLTYAVNIWSENDPADFRIYNVTYLEPSLRIAASTLKSGISYRARVRAWAQCYNTTWSEWSPSTKWHNSYREPFEQ
-
蛋白长度
Extracellular Domain
-
分子量
60-70 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Related Products
Protein Description
Interleukin-4 receptor alpha (IL-4RA) is a key component of the immune response, primarily mediating the effects of interleukin-4 (IL-4) and interleukin-13 (IL-13), two critical cytokines involved in allergic reactions, asthma, and various immune disorders. The IL-4RA receptor is involved in the differentiation and proliferation of immune cells, particularly T-helper 2 (Th2) cells, which are pivotal in driving allergic inflammation. Dysregulation of IL-4RA signaling has been implicated in the pathogenesis of several diseases, including asthma, atopic dermatitis, and chronic rhinosinusitis. Understanding the structure and function of IL-4RA is crucial for the development of targeted therapies aimed at modulating immune responses. Researchers are exploring recombinant IL-4RA proteins to elucidate the receptor's biological pathways, assess its potential as a therapeutic target, and improve our understanding of Th2-mediated conditions. These studies may pave the way for innovative treatment strategies that could mitigate the impact of IL-4RA-related diseases, providing hope for more effective management of allergic conditions through the modulation of IL-4 signaling pathways.
