Analytical Data
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Gene name
SPINK5
- Application
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Alternative Names
SPINK5；Serine protease inhibitor Kazal-type 5
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NQ38
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Expression Region
722-847aa
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AA Sequence
TRESDPVRDADGKSYNNQCTMCKAKLEREAERKNEYSRSRSNGTGSESGKDTCDEFRSQMKNGKLICTRESDPVRGPDGKTHGNKCTMCKEKLEREAAEKKKKEDEDRSNTGERSNTGERSNDKED
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Molecular Weight
49.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SPINK5, also known as Serine Peptidase Inhibitor, Kazal Type 5, encodes a protein that plays a critical role in skin barrier function and immune response. Mutations in the SPINK5 gene have been linked to various skin disorders, most notably Netherton syndrome, which is characterized by atopic dermatitis, hair shaft defects, and immunological issues. Research on SPINK5 recombinant protein focuses on understanding its structural and functional properties, as well as its interactions with serine proteases, which are implicated in inflammation and skin barrier integrity. By expressing and purifying SPINK5 as a recombinant protein, researchers aim to elucidate its mechanisms of action at a molecular level, potentially paving the way for novel therapeutic strategies for skin conditions linked to SPINK5 deficiencies. Additionally, studying this protein can offer insights into the broader context of epidermal homeostasis and its implications in dermatological health. The increasing interest in protein-based therapies and bioengineering further highlights the significance of SPINK5 research in developing targeted treatments, thereby improving the quality of life for patients with related skin diseases.
