Analytical Data
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Gene name
DGUOK
- Application
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Alternative Names
DGUOK; DGK; Deoxyguanosine kinase; mitochondrial; dGK; EC 2.7.1.113
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Species
Human
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Source
E. coli
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Tag
N- His-SUMO
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q16854
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Expression Region
40-277aa
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Molecular Weight
44 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DGUOK (Deoxyguanosine Kinase) is a key enzyme in the purine nucleotide salvage pathway, responsible for phosphorylating deoxyguanosine to deoxyguanosine monophosphate (dGMP), which is essential for DNA synthesis and repair. Mutations or dysfunctions in the DGUOK gene have been associated with severe mitochondrial diseases, particularly those affecting the brain and muscle tissues, leading to energy deficits and cellular dysfunction. Due to its vital role in nucleotide metabolism, the study of DGUOK presents a significant interest in the fields of molecular biology, genetics, and therapeutic development. The recombinant expression of DGUOK provides a powerful tool to better understand its enzymatic function, interactions with substrates and inhibitors, and potential as a target for drug development in diseases related to nucleotide metabolism. Researchers are focusing on characterizing the properties of DGUOK, exploring its structure-function relationships, and devising gene therapy strategies that target DGUOK deficiencies. This includes producing high-yield recombinant DGUOK proteins for structure elucidation through X-ray crystallography or cryo-electron microscopy, which can shed light on its catalytic mechanisms and regulation. Ultimately, advancements in DGUOK research could pave the way for novel therapeutic approaches to combat disorders stemming from its dysfunction, emphasizing the translational importance of this enzyme in both basic and clinical research.
