Analytical Data
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Gene name
ORF1a
- Application
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Alternative Names
/
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Species
Cucumber mosaic virus
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Source
E. coli
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Tag
C- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
K8DRS1
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Expression Region
2-302aa
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Molecular Weight
37.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The ORF1a protein, an essential component of the viral replication machinery in coronaviruses, has garnered significant attention in recent years due to its critical role in the life cycle of these viruses, including SARS-CoV-2, which is responsible for the COVID-19 pandemic. ORF1a encodes polyproteins that are cleaved into functional non-structural proteins, contributing to viral replication and transcription. Studies have highlighted the intricate mechanisms by which ORF1a interacts with host cell factors, facilitating viral adaptation and evasion of the host immune response. Understanding the structure and function of ORF1a is crucial for the development of antiviral therapeutics and vaccines. Recent research has focused on the characterization of recombinant ORF1a proteins, which serve as key tools for studying viral biology and testing potential inhibitors of viral replication. The insights gained from these studies are not only vital for combating current coronavirus outbreaks but also for preparing for future zoonotic spillovers and pandemics.
