Analytical Data
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Gene name
ASF1B
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简介
The ASF1B protein is an important histone chaperone that crucially promotes histone deposition and coordinates exchange and removal during nucleosome assembly and disassembly, which has been verified by various studies. ASF1B cooperates with CAF-1 to promote replication-dependent chromatin assembly. ASF1B Protein, Human (sf9, His) is the recombinant human-derived ASF1B protein, expressed by Sf9 insect cells , with C-His labeled tag.
- Application
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Alternative Names
Histone chaperone ASF1B; hAsf1b; CCG1-interacting factor A-II; CIA-II
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Species
Human
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Source
Baculovirus
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9NVP2
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Expression Region
M1-I202
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Protein Length
Full Length
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Molecular Weight
20 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ASF1B (Asf1 Histone Chaperone B) is a vital protein involved in chromatin remodeling and histone management during DNA replication and repair. It functions as a histone chaperone, facilitating the assembly and disassembly of histone proteins on DNA, thus playing a crucial role in maintaining genome stability and regulating gene expression. Research into ASF1B has gained significant attention due to its implications in various biological processes and its potential links to cancer. Aberrant expression of ASF1B has been associated with several tumor types, suggesting that it could serve as a biomarker for cancer progression or a therapeutic target. Recent studies have focused on understanding the structural characteristics of ASF1B, its interaction with other chromatin-associated factors, and the mechanisms through which it contributes to DNA damage response and repair. The ability to produce recombinant ASF1B protein has facilitated these investigations, enabling detailed biochemical and biophysical studies that illuminate its functional properties. Understanding the precise role of ASF1B in cellular processes not only enhances our knowledge of epigenetic regulation but also opens up avenues for therapeutic interventions in diseases linked to chromatin dysregulation. The ongoing research into ASF1B is pivotal for uncovering the complexities of chromatin dynamics and its impact on cellular health and disease.
