Analytical Data
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Gene name
SIRP beta 1/CD172b
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简介
SIRP beta 1 protein negatively regulates receptor tyrosine kinase signaling and recruits SYK for myeloid cell activation. It interacts with TYROBP as a homodimer, facilitating SYK recruitment. This intricate molecular interplay is crucial for modulating myeloid cell activation and regulating cellular signaling. SIRP beta 1 occupies a central position in protein interactions that contribute to its functional role. SIRP Beta 1 Protein, Human (Biotinylated, HEK293, C-His-Avi) is the recombinant human-derived SIRP Beta 1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
- Application
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Alternative Names
SIRP-beta-1 isoform 3; SIRPB1; CD172b; DKFZp686A05192; FLJ26614; 9930027N05Rik; SIRP-beta; Sirpb; Sirpb1; Sirpb1a; SIRP-β-1 isoform 3; SIRP-β
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Species
Human
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Source
HEK293
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Tag
C-Avi;C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00241-1
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Expression Region
E30-A369
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Protein Length
Partial
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Molecular Weight
55-65 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SIRP beta 1, also known as CD172b, is an immunoglobulin-like receptor primarily expressed in myeloid cells, including macrophages and dendritic cells. It plays a crucial role in regulating immune responses, particularly by modulating the interaction between immune cells and their environment. The SIRP/CD47 signaling axis has garnered significant interest in cancer immunotherapy, as CD47, often overexpressed on tumor cells, acts as a "don't eat me" signal, inhibiting phagocytosis by macrophages. By targeting the SIRP beta 1/CD47 pathway, researchers aim to enhance the ability of the immune system to recognize and eliminate cancer cells. Recent studies have indicated that recombinant forms of SIRP beta 1 can block CD47 signaling, promoting the phagocytosis of tumor cells and potentially improving therapeutic efficacy. Furthermore, understanding the structural and functional properties of SIRP beta 1 is essential for the development of novel immunotherapeutic strategies. Hence, characterizing the recombinant SIRP beta 1/CD172b protein is a pivotal step towards elucidating its mechanisms and reinforcing its role as a target in cancer therapy. Researchers are exploring its interactions with various ligands and its downstream signaling pathways, which may reveal new insights into the modulation of immune responses and the design of more effective treatments for cancers and immune-related diseases.
