Analytical Data
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Gene name
PGD
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简介
PGD Protein is crucial in cellular metabolism, catalyzing the oxidative decarboxylation of 6-phosphogluconate. This process yields ribulose 5-phosphate, releases CO(2), and simultaneously reduces NADP to NADPH, contributing to essential cellular redox balance. The enzyme's functionality underscores its significance in maintaining cellular homeostasis and participating in key metabolic pathways. PGD Protein, Human (His) is the recombinant human-derived PGD protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
6-phosphogluconate dehydrogenase, decarboxylating; PGD; PGDH
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P52209-1
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Expression Region
M1-A483
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Protein Length
Full Length of Isoform-1
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Molecular Weight
46 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PGD (Phosphogluconate Dehydrogenase) recombinant proteins have garnered significant attention in biochemical and medical research due to their crucial role in the pentose phosphate pathway (PPP). This metabolic pathway is essential for cellular functions, including the generation of NADPH, which is vital for biosynthetic reactions and maintaining cellular redox balance, as well as providing ribose-5-phosphate for nucleotide synthesis. Alterations in PGD activity have been linked to various diseases, including cancer and metabolic disorders, highlighting the importance of understanding its function and regulation. Advances in recombinant DNA technology have enabled the production of PGD in heterologous systems, allowing for the detailed study of its enzymatic properties, structural characteristics, and interactions with other metabolic pathways. Research on PGD recombinant proteins not only enhances our understanding of fundamental metabolic processes but also paves the way for potential therapeutic strategies aimed at targeting PGD-related pathways in disease contexts. Moreover, the ability to produce PGD in large quantities facilitates the development of diagnostic tools and biotechnological applications, underscoring the relevance of PGD in both basic and applied sciences. Consequently, the exploration of PGD recombinant proteins remains a vibrant field of study with implications spanning from molecular biology to clinical applications.
