Analytical Data
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Gene name
Semaphorin-4D/SEMA4D
- Application
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Alternative Names
CD100; A8; BB18; GR3; M-Sema G; M-Sema-G; SEMAJ; Coll-4; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D
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Species
Mouse
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
O09126
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Expression Region
Phe502~Ser636
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Molecular Weight
16kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Semaphorin-4D (SEMA4D), a member of the semaphorin family, plays a critical role in various physiological and pathological processes, including immune response, neuronal development, and tumor progression. It functions primarily through its interaction with neuropilin and plexin receptors, influencing cell migration, proliferation, and apoptosis. Research has increasingly focused on the role of SEMA4D in cancer biology, where it has been shown to promote tumor growth, metastasis, and immune evasion by modulating the tumor microenvironment and inhibiting anti-tumor immunity. The overexpression of SEMA4D in several malignancies, such as breast cancer and melanoma, highlights its potential as a therapeutic target. Given its involvement in essential signaling pathways, recombinant SEMA4D proteins have become valuable tools for studying these mechanisms. Moreover, understanding the biological effects of SEMA4D through recombinant proteins can lead to novel therapeutic strategies, including blocking SEMA4D's interactions to enhance anti-tumor immunity and inhibit cancer progression. These insights could pave the way for innovative treatments, particularly in combination with existing immunotherapies. As research continues to elucidate the complex roles of SEMA4D, it remains a promising candidate for targeted therapies aimed at manipulating immune responses and improving clinical outcomes in cancer patients.
