Analytical Data
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Gene name
Hyaluronan Lyase
- Application
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Alternative Names
Hyaluronidase; SAG1197
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Species
Others
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
NP_688206
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Expression Region
S259-I1072
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AA Sequence
SEHPQPVTTQIEKSVNTALNKNYVFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTASTEDNFTKLLDKWNDVTIGNHVYDTNDSNMQKLNQKLDETNAKNIEAIKLDSNRTFLWKDLDNLNNSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVNKDIEGSANWWDFEIGVPRSITGTLALMYNYFTDAEIKTYTDPIEHFVPDAGFFRKTLVNPFKALGGNLVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYIDHTNVAYTGAYGNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELMDMSRGRSISREAASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVFNNLKSYSDIANMNKLLNDSTVATKPLKSNLSTFNSMDRLAYYNAEKDFGFALSLHSKRTLNYEGMNDENTRGWYTGDGMFYLYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDTTKDFMSKHSKDAKEKTGQVTGASDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVILNDKIVFLGSNIKNTNGIGNVSTTIDQRKDDSKTPYTTYVNGKTVDLKQASSQQFTDTKSVFLESKEPGRNIGYIFFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKHDNKGDSYDYMMVPNIDRTSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQESLINNQFKMNKAGLYLVQKVGNDYQNVYYQPQSMTKTDQLAI
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Protein Length
Partial
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Molecular Weight
85 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Hyaluronan lyase (Hyalase) is an enzyme that catalyzes the cleavage of hyaluronan, a glycosaminoglycan present in connective tissues, skin, and various bodily fluids, into smaller fragments. This enzyme plays a crucial role in various biological processes, including tissue remodeling, cell migration, and inflammation. The interest in recombinant hyaluronan lyase has surged due to its potential applications in biomedicine and biotechnology, particularly in wound healing, drug delivery, and cancer therapy, where the modulation of the extracellular matrix is vital. Traditional methods for obtaining hyaluronan lyase often involve complex and inefficient extraction processes from bacterial or animal sources. However, recombinant DNA technology allows for the production of this enzyme in a controlled environment, ensuring higher yields and purity, which are essential for therapeutic applications. Research efforts have focused on optimizing the expression and purification of recombinant hyaluronan lyase, as well as characterizing its biochemical properties and substrate specificity. Additionally, studies are investigating the enzyme's role in pathophysiological conditions, such as osteoarthritis and tumor progression, highlighting its importance in understanding disease mechanisms and developing targeted therapies. The growing body of research emphasizes the need for efficient recombinant production methods and deepens our understanding of hyaluronan metabolism, paving the way for innovative therapeutic strategies in regenerative medicine and oncology. Overall, hyaluronan lyase represents a fascinating target for research due to its significant implications in health and disease, making it a valuable subject of scientific inquiry.
