Analytical Data
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Gene name
Norrin
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简介
Norrin protein is a key component of the canonical Wnt signaling pathway, activating the cascade by interacting with the FZD4 receptor and LRP5 coreceptor. Its role in retinal vascularization includes serving as a FZD4 ligand, stabilizing β-catenin (CTNNB1), and initiating LEF/TCF-mediated transcription. Norrin Protein, Mouse is the recombinant mouse-derived Norrin protein, expressed by E. coli , with tag free.
- Application
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Biological Activity
Measured by its binding ability in a functional ELISA. When Mouse Norrin is coated at 0.5 μg/mL (100 μL/well) can bind Human Frizzled4. The ED50 for this effect is 84.14 ng/mL. Measured by its binding ability in a functional ELISA. When Mouse Norrin is coated at 0.5 μg/mL (100 μL/well) can bind Human Frizzled4. The ED50 for this effect is 84.14 ng/mL.
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Alternative Names
Norrin; Ndp; Norrie disease protein homolog; Ndph
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Species
Mouse
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P48744
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Expression Region
K25-S131
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Protein Length
Full Length of Mature Protein
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Molecular Weight
13 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Norrin is a secreted cysteine-rich growth factor that primarily plays a pivotal role in the development and maintenance of the retinal vasculature and the inner ear. It is encoded by the NDP gene, and mutations in this gene are implicated in Norrie disease, a rare genetic disorder leading to vision and hearing loss. Understanding the biological functions and mechanisms of Norrin is crucial for elucidating its role in angiogenesis and neural development. Recent studies have highlighted its interactions with Frizzled-4 and β-catenin in the Wnt signaling pathway, which are essential for endothelial cell communication and blood-brain barrier integrity. Given its significance in both normal physiological processes and disease states, characterizing Norrin as a recombinant protein is essential for exploring therapeutic applications. Researchers have been focusing on producing recombinant Norrin to investigate its structural properties, biological activities, and potential as a therapeutic agent for ocular and auditory disorders. By utilizing advanced protein engineering techniques, scientists aim to enhance the understanding of Norrin's functional mechanisms and develop targeted interventions that could mitigate the effects of Norrie disease and similar conditions. As studies progress, the promise of recombinant Norrin in regenerative medicine and its implications for treating vascular and sensory disorders continue to garner attention in the biomedical research community.
