Analytical Data
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基因名
COL4A1
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简介
COL4A1 protein is an important type IV collagen component that forms the main structural framework of the glomerular basement membrane (GBM) with a unique "chicken wire" mesh structure. It cooperates with laminin, proteoglycans and nestin/nesidin to maintain the structural integrity of GBM. COL4A1 Protein, Human (His) is the recombinant human-derived COL4A1 protein, expressed by E. coli, with C-His labeled tag.
- Application
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别名
Arresten; BSVD; COL4A1; COL4A1 NC1 domain; COL4A2; COL4A3; COL4A4; COL4A5; collagen alpha-1(IV) chain
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种属
Human
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表达系统
E. coli
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标签
C-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P02462
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表达区间
G30-P167
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蛋白长度
Partial
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分子量
25 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
COL4A1 is a gene that encodes one of the collagen type IV chains, which are crucial components of basement membranes, providing structural support and maintaining tissue integrity. Mutations in COL4A1 have been linked to various diseases, including cerebrovascular abnormalities, kidney disease, and muscular dystrophy. The study of COL4A1 recombinant protein is essential for understanding the functional roles of this collagen in physiological and pathological processes. Researchers have focused on producing recombinant COL4A1 to investigate its molecular interactions, structural properties, and potential therapeutic applications. By using techniques such as recombinant DNA technology and protein expression systems, scientists can generate large quantities of COL4A1 protein to study its biological significance in both health and disease. This research not only contributes to our understanding of collagen-related disorders but also paves the way for developing targeted therapies that may mitigate the effects of COL4A1 mutations. Additionally, exploring the properties of COL4A1 can enhance tissue engineering approaches, where collagen and its derivatives are increasingly used to create biomaterials for regenerative medicine. Overall, the investigation of COL4A1 recombinant protein is a promising avenue for advancing both fundamental research and clinical applications in the field of collagen-associated diseases.
