Analytical Data
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Gene name
PDHa2
- Application
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Alternative Names
PDHAL; Lipoamide; Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P29803
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Expression Region
Ser28~Ser388
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Molecular Weight
44kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PDHa2 (Pyruvate Dehydrogenase E2) is a crucial enzyme in cellular metabolism, specifically in the pyruvate dehydrogenase complex, which plays a pivotal role in converting pyruvate into acetyl-CoA, a key intermediate in energy production and various biosynthetic pathways. Dysregulation of PDHa2 has been implicated in several metabolic disorders, including obesity, type 2 diabetes, and certain types of cancer. As such, the study of PDHa2 recombinant proteins has gained significant attention as researchers aim to understand its molecular mechanisms and regulatory pathways. Recombinant PDHa2 proteins enable detailed structural and functional analyses, helping to elucidate how post-translational modifications and enzyme activity influence metabolic pathways. Additionally, these studies provide insights into potential therapeutic targets for metabolic diseases. By employing techniques such as crystallography and mass spectrometry, researchers can investigate the enzyme's interactions and dynamics within the pyruvate dehydrogenase complex, fostering the development of innovative strategies for the treatment of related health conditions. Overall, PDHa2 research is essential for advancing our comprehension of metabolic regulation and disease, highlighting its relevance in both basic research and clinical applications.
