Analytical Data
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Gene name
HNF4A
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简介
HNF 4 alpha, a nuclear transcription factor, forms homodimers to bind DNA. It regulates gene expression, including hepatocyte nuclear factor 1 alpha, impacting hepatic genes. Its involvement extends to liver, kidney, and intestinal development. Mutations associate with autosomal dominant non-insulin-dependent diabetes mellitus type I. The gene exhibits biased expression in the duodenum (RPKM 37.7) and colon (RPKM 32.8), among other tissues. HNF4A Protein, Human (HEK293, His) is the recombinant human-derived HNF 4 alpha, expressed by HEK293 , with C-10*His labeled tag.
- Application
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Alternative Names
FRTS4; HNF4; HNF4a7; HNF4a8; HNF4a9; HNF4alpha; MODY; MODY1; NR2A1; NR2A21; TCF; TCF-14; TCF14
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Species
Human
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Source
HEK293
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Tag
C-10*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P41235-1
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Expression Region
M1-I474
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AA Sequence
MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI
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Protein Length
Full Length of Isoform-1
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Molecular Weight
80 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
HNF4A (Hepatocyte Nuclear Factor 4 Alpha) is a key transcription factor that plays a critical role in the regulation of genes involved in glucose and lipid metabolism, as well as in maintaining liver and intestinal function. Mutations in the HNF4A gene are associated with various metabolic disorders, particularly maturity-onset diabetes of the young (MODY) and certain forms of hyperlipidemia. Given its pivotal role in metabolic processes, the study of HNF4A has garnered considerable interest in biomedical research. Recombinant HNF4A protein serves as a valuable tool for elucidating its functional mechanisms and downstream signaling pathways. By producing and purifying HNF4A in vitro, researchers can investigate its DNA-binding properties, transcriptional activation capabilities, and interactions with other proteins. Additionally, recombinant HNF4A enables the development of potential therapeutic strategies to treat metabolic diseases caused by its dysfunction. Understanding the structure-function relationship of HNF4A through recombinant approaches can reveal insights into its regulatory roles and contribute to the design of small molecules or gene therapies aimed at restoring HNF4A function in affected individuals. As research advances, the therapeutic potential of targeting HNF4A continues to be explored, making studies on recombinant HNF4A not only relevant for basic science but also for clinical applications in metabolic disorder management.
