Analytical Data
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Gene name
DDR1/CD167a
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简介
The DDR1 protein is a tyrosine kinase and cell surface receptor for fibrillar collagen that tightly regulates cell attachment and affects extracellular matrix processes. Upon collagen binding, DDR1 initiates a signaling cascade involving SRC, activating MAP kinase. DDR1 Protein, Human (HEK293, His) is the recombinant human-derived DDR1 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
1. Immobilized Human DDR1, His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of <12.8 ng/mL determined by ELISA . 2. Human DDR1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.806 nM as determined in SPR assay (Biacore T200). 3.Immobilized Human DDR1, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 12.5ng/ml determined by ELISA
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Alternative Names
Epithelial discoidin domain-containing receptor 1; HGK2; CD167a; CAK; EDDR1
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Species
Human
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Source
HEK293
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Tag
C-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q08345-1
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Expression Region
D21-A417
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Protein Length
Extracellular Domain
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Molecular Weight
55-70 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
DDR1, also known as CD167a, is a receptor tyrosine kinase that plays a crucial role in various cell processes such as migration, differentiation, and tissue remodeling, particularly in the context of the extracellular matrix. It is primarily expressed in cells of mesenchymal origin and is implicated in several pathological conditions, including cancer, fibrosis, and inflammatory diseases. The study of DDR1 has gained attention due to its involvement in the tumor microenvironment, where it is believed to influence tumor progression and metastasis. Research on recombinant DDR1/CD167a proteins has been pivotal in understanding its structural and functional properties, providing insights into its ligand-binding mechanisms and signaling pathways. By creating these recombinant proteins, scientists can explore the receptor's interactions with ligands like collagen, analyze its role in cellular signaling, and evaluate its potential as a therapeutic target. Furthermore, understanding the biophysical properties of DDR1 reaffirms its significance in cell behavior and offers opportunities for the development of novel treatment strategies aimed at modulating its activity in disease contexts. Consequently, the exploration of DDR1/CD167a recombinant proteins is not only essential for elucidating its biological functions but also for the advancement of targeted therapies aimed at various diseases linked to this receptor.
