Analytical Data
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Gene name
MCT/SACOL1244
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简介
MCT/SACOL1244 Protein intricately participates in lipid metabolism, specifically playing a vital role in fatty acid biosynthesis. MCT/SACOL1244 Protein, S. aureus (P.pastoris, His) is the recombinant Staphylococcus aureus-derived MCT/SACOL1244 protein, expressed by P. pastoris , with N-6*His labeled tag.
- Application
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Alternative Names
fabD; SACOL1244Malonyl CoA-acyl carrier protein transacylase; MCT; EC 2.3.1.39
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Species
Staphylococcus aureus
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Source
P. pastoris
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q5HGK3
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Expression Region
M1-D308
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AA Sequence
MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDILETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVLSFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANINCPGQIVVSGHKALIDELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFSSYINQFEWRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPGKVLSGLIKKINRDVKLTSIQTLEDVKGWNEND
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Protein Length
Full Length
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Molecular Weight
35.6 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MCT (Monocarboxylate Transporter) and SACOL1244 are key proteins involved in the metabolic processes of various organisms, including bacteria, plants, and animals. Recent research has highlighted the importance of MCTs in transporting lactate, pyruvate, and other monocarboxylates across cellular membranes, playing a crucial role in energy metabolism, pH regulation, and cell signaling. Specifically, SACOL1244, a member of the MCT family, is of particular interest due to its potential involvement in applications ranging from biotechnology to medicine. Understanding the structure and function of this recombinant protein can provide insights into its transport mechanisms and physiological roles. Researchers have been focusing on the recombinant expression of SACOL1244 to facilitate detailed studies of its biophysical properties, transport kinetics, and interactions with various substrates. This research is not only significant for advancing our basic understanding of cellular transport mechanisms but also holds promise for developing targeted therapeutic strategies in metabolic disorders where MCT dysfunction is implicated. By elucidating the characteristics and functions of SACOL1244, researchers aim to harness its potential for improving metabolic health and inform future biotechnological applications.
