Analytical Data
-
Gene name
CCL4
-
简介
CCL4 Protein, Human (P.pastoris, His) is a small cytokine of the CC chemokine subfamily that binds to the CCR5 chemokine receptor on the cell surface, promotes leukocyte aggregation under various inflammatory conditions, and contributes to immune protection against human immunodeficiency virus type 1. CCL4 Protein, Human (P.pastoris, His) is a recombinant human CCL4 (A24-Q92) expressed by P. pastoris with a His tag at the C-terminu[1].
- Application
-
Alternative Names
MIP-1β/CCL4; C-C motif chemokine 4; LAG-1; SCYA4
-
Species
Human
-
Source
P. pastoris
-
Tag
C-His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
P13236
-
Expression Region
A24-Q92
-
Protein Length
Full Length of Mature Protein
-
Molecular Weight
12-16 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CCL4 (Chemokine C-C motif ligand 4), also known as MIP-1β (macrophage inflammatory protein-1 beta), is an important chemokine involved in immune responses. It plays a significant role in the recruitment and activation of immune cells, particularly T cells and macrophages, to sites of inflammation and infection. Research on CCL4 has gained momentum due to its involvement in various pathological conditions, including autoimmune diseases, cancer, and viral infections. The understanding of CCL4's function is critical for developing therapeutic strategies aimed at modulating immune responses. Recombinant CCL4 proteins have been developed for experimental and therapeutic purposes, facilitating the investigation of its roles in immune signaling pathways. The study of CCL4's structure and function is essential in elucidating its interactions with specific receptors, such as CCR5, which is a co-receptor for HIV entry into host cells. Furthermore, recombinant CCL4 is utilized in assays to assess its effects on leukocyte migration, proliferation, and activation, offering insights into its potential as a therapeutic target. Given its multifunctional properties in the immune system, CCL4 continues to be a subject of intense study, with the aim of leveraging its biological activities to enhance vaccine efficacy, improve anti-tumor responses, and develop novel therapies for various diseases.
