Analytical Data
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Gene name
rpsD
- Application
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Alternative Names
rpsD; ramA; b3296; JW3258; 30S ribosomal protein S4; Small ribosomal subunit protein uS4
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Species
Escherichia coli
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Source
E. coli
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P0A7V8
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Expression Region
2-206aa
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Molecular Weight
27.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The rpsD gene encodes the ribosomal protein S4, a crucial component of the 30S ribosomal subunit in prokaryotic organisms. This protein plays a vital role in ribosome assembly and function, influencing both translation accuracy and efficiency. Studies of rpsD and its encoded protein have significant implications for understanding bacterial growth and metabolism, as well as for the development of novel antimicrobial strategies. The ribosomal protein S4 is highly conserved across various species, making it an excellent model for studying ribosomal structure and function. Additionally, due to its central role in protein synthesis, rpsD presents potential as a target for antibiotic development. Research has shown that mutations or alterations in rpsD can lead to antibiotic resistance, highlighting the importance of this protein in both fundamental biology and public health. The reconstituted or recombinant versions of rpsD protein allow scientists to explore its interactions with other ribosomal components and its role in the translation process in more detail. These investigations contribute to a broader understanding of ribosomal mechanics and can provide insights into the adaptation mechanisms of bacteria under stress or during antibiotic exposure. By unraveling the complexities of rpsD function and its implications in bacterial physiology, researchers aim to not only elucidate fundamental biological processes but also to foster the discovery of innovative antimicrobial therapies to combat resistant bacterial strains. Thus, the study of rpsD recombinant protein is at the intersection of microbiology, molecular biology, and therapeutic development, signifying its crucial role in advancing both scientific knowledge and public health outcomes.
