Analytical Data
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Gene name
FGFR-4
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简介
The FGFR-4 protein is a tyrosine-protein kinase receptor for fibroblast growth factor that regulates cellular processes, lipid metabolism, bile acid biosynthesis, and more.Its effects include downregulation of CYP7A1 in response to FGF19.FGFR-4 Protein, Rat (HEK293, C-hFc) is the recombinant rat-derived FGFR-4 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Biological Activity
1. Measured by its binding ability in a functional ELISA. 2. Immobilized human FGF18 at 10 μg/mL (100 μl/well) can bind Rat FGFR4 , The EC50 of Rat FGFR4 is <1.17 μg/mL. 3.Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 1.025 ng/mL, corresponding to a specific activity is 9.76×10^5 units/mg. Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED50 for this effect is 1.025 ng/mL, corresponding to a specific activity is 9.76×105 units/mg.
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Alternative Names
Fibroblast growth factor receptor 4; FGFR-4; CD334; JTK2; TKF
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Species
Rat
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q498D6
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Expression Region
F17-D367
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AA Sequence
FSLEASEEMEQEPCPAPISEQQEQVLTVALGQPVRLCCGRTERGRHWYKEGSRLASAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMTVVHNLTLIMDDSLPSINNEDPKTLSSSSSGHSYLQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPMPTIHWLKNGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENSLGSIRYSYLLDVLERSPHRPILQAGLPANTTAVVGSNVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTTDINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPAEEEDLAWTTATSEARYTD
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Protein Length
Extracellular Domain
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Molecular Weight
110 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Fibroblast growth factor receptor 4 (FGFR-4) is a pivotal member of the fibroblast growth factor receptor family, involved in various physiological processes, including cell proliferation, differentiation, and survival. Research has demonstrated that FGFR-4 plays a critical role in several pathological conditions, including cancer, where its aberrant expression is linked to tumor progression and metastatic potential. Understanding the structural and functional dynamics of FGFR-4 has become essential for developing targeted therapies against cancers associated with its dysregulation. Recombinant FGFR-4 proteins are utilized in studies to elucidate the receptor's signaling mechanisms, interactions with ligands, and downstream effectors. These proteins can also aid in high-throughput screening for novel inhibitors that may serve as potential therapeutic agents. Moreover, FGFR-4 is implicated in muscle development and repair, highlighting its importance beyond oncology. By producing and characterizing FGFR-4 recombinant proteins, researchers aim to gain insights into its biological roles and therapeutic targeting, paving the way for innovative treatment strategies for diseases linked to FGFR-4 dysregulation.
