Analytical Data
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Gene name
MIP-1 alpha/CCL3
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简介
MIP-1 alpha/CCL3 Protein, Mouse (His), an important chemokine, is a key regulator of immune microenvironment and primarily mediates the trafficking of immune cells in both inflammation and cancer. MIP-1 alpha/CCL3 Protein, Mouse (His) is a recombinant mouse CCL3 (A24-A92) expressed by E.coil with a N-6*His tag[1].
- Application
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Biological Activity
Measured by its ability to chemoattract BaF3 mouse proB cells transfected with mouse CCR5. The ED50 for this effect is <3.0 ng/mL. Measured by its ability to chemoattract BaF3 mouse proB cells transfected with mouse CCR5. The ED50 for this effect is 2.771 ng/mL, corresponding to a specific activity is 3.609×105 U/mg.
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Alternative Names
rMuCCL3, His; C-C motif chemokine 3; Heparin-binding chemotaxis protein; LD78 alpha; MIP-1 alpha; SCYA3; CCL3
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Species
Mouse
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P10855
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Expression Region
A24-A92
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Protein Length
Full Length of Mature Protein
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Molecular Weight
12-15 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
MIP-1 alpha, also known as CCL3 (chemokine (C-C motif) ligand 3), is a key chemokine involved in immune responses, particularly in the recruitment and activation of leukocytes during inflammation and infection. Research into CCL3 has expanded due to its significant role in various pathological conditions, including autoimmune diseases, viral infections, and cancer. The recombinant form of MIP-1 alpha is produced through genetic engineering techniques, which facilitate the study of its functions and potential therapeutic applications. Studies have shown that CCL3 influences the migration of T cells and monocytes to inflammatory sites, modulating the immune response. Moreover, its expression is often upregulated in diseases where the immune system is dysregulated. Understanding the signaling pathways and biological activities of recombinant CCL3 can provide valuable insights into its potential as a biomarker for disease progression and as a target for therapeutic intervention. Current research explores its therapeutic implications in enhancing immune response against tumors and addressing inflammatory diseases, making recombinant MIP-1 alpha a pivotal molecule in immunological studies.
