Analytical Data
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Gene name
SMAD9
- Application
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Alternative Names
MADH2; MADR2; Madh6; Mothers against DPP homolog 9; Mothers Against Decapentaplegic Homolog 9
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Species
Human
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Source
E. coli
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Tag
N- His & GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O15198
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Expression Region
Thr226~Pro459
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Molecular Weight
56kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SMAD9, a member of the SMAD family of proteins, plays a crucial role in the transforming growth factor-beta (TGF-β) signaling pathway, which is essential for various cellular processes, including cell differentiation, proliferation, and apoptosis. Research into SMAD9 has gained significant interest due to its involvement in diverse physiological and pathological conditions, such as embryonic development, tissue homeostasis, and cancer progression. As a receptor-regulated SMAD, SMAD9 transmits signals from TGF-β receptors to the nucleus, where it regulates the expression of target genes. The study of SMAD9 recombinant proteins has become increasingly important for understanding the molecular mechanisms underlying these processes. By producing and characterizing SMAD9 in a recombinant form, researchers can investigate its functional properties, interactions with other signaling molecules, and its influence on downstream gene regulation. Furthermore, recombinant SMAD9 can be used as a valuable tool in drug discovery, allowing the identification of compounds that modulate its activity and potentially targeting TGF-β signaling pathways for therapeutic interventions. Given the complexity of TGF-β signaling and its implications in various diseases, the ongoing research on SMAD9 not only enhances our understanding of cell signaling but also paves the way for novel clinical applications in the treatment of cancer and fibrotic diseases.
