Analytical Data
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基因名
POLR1D
- Application
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别名
AC19 DNA-directed RNA polymerase I subunit D RNA polymerase I 16KDA subunit
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种属
Human
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表达系统
E. coli
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标签
N- GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y2S0
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表达区间
1-133aa
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分子量
42.2 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
POLR1D, or RNA polymerase I subunit D, is a crucial component of the RNA polymerase I complex, which is responsible for synthesizing ribosomal RNA (rRNA) in eukaryotic cells. The ribosomal RNA is fundamental for ribosome biogenesis and, consequently, for protein synthesis, making POLR1D vital for cellular growth and proliferation. Research into the POLR1D recombinant protein has gained significant traction due to its potential implications in understanding various diseases, including cancer. Dysregulation of ribosome biogenesis is often associated with oncogenesis, and several studies have indicated that alterations in RNA polymerase activity can affect tumor growth and stability. Investigating the structure, function, and interactions of the POLR1D protein is essential for deciphering its role in both normal and pathological states. Recombinant POLR1D offers opportunities to study its biochemical properties and its interactions with other proteins within the ribosome synthesis pathway, thus providing insights into fundamental cellular mechanisms. This research not only contributes to our understanding of rRNA synthesis but also paves the way for potential therapeutic targets in diseases linked to ribosome biogenesis dysfunction. As the field progresses, elucidating the precise mechanisms and regulatory roles of POLR1D will be vital for advancing our knowledge of cell biology and developing novel interventions for related diseases.
