Analytical Data
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Gene name
RAPGEF1
- Application
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Alternative Names
C3G; GRF2; RAP-GEF1; Guanine Nucleotide-Releasing Factor 2(Specific For Crk Proto-Oncogene); CRK SH3-binding GNRP
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13905
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Expression Region
Lys780~Asn1035
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Molecular Weight
34kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
RAPGEF1, a member of the Ras activator protein family, plays a crucial role in cellular signaling pathways by activating the Ras subfamily of GTPases. It has been implicated in various physiological processes, including cell proliferation, differentiation, and survival. Research into RAPGEF1 is particularly relevant due to its involvement in several cancers and other diseases, where aberrant Ras signaling can contribute to tumorigenesis. The understanding of RAPGEF1's structure and function has been advanced through recombinant protein studies, which allow for the detailed analysis of its enzymatic activity and interaction with Ras proteins. Additionally, the generation of RAPGEF1 recombinant proteins enables researchers to explore its regulatory mechanisms and potential as a therapeutic target. Given its pivotal role in signaling cascades, RAPGEF1 is an attractive candidate for drug development, aiming to inhibit its activity or enhance its function in disease contexts. Overall, the study of RAPGEF1 and its recombinant forms is crucial for elucidating the complexities of Ras-mediated signaling and its implications in health and disease.
