Analytical Data
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Gene name
DNM2
- Application
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Alternative Names
CMTDI1; CMTDIB; DYN2; DYNII; Cytoskeletal Protein
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P50570
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Expression Region
Leu533~Glu731
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Molecular Weight
27kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Dynamin-2 (DNM2) is a critical member of the dynamin family of GTPases, which play a vital role in membrane dynamics and intracellular trafficking. As a large GTPase, DNM2 is primarily involved in endocytosis, particularly in clathrin-mediated and caveolae-dependent pathways. Mutations and dysregulation of DNM2 have been implicated in various diseases, including neurological disorders and muscular dystrophies, highlighting its importance in cellular physiology and pathology. The study of recombinant DNM2 proteins has gained traction as researchers aim to elucidate its mechanistic functions at the molecular level. By expressing and purifying recombinant DNM2, scientists can investigate its GTPase activity, oligomerization behavior, and interaction with membrane systems, providing insights into the conformational changes that occur during its functional cycle. Furthermore, understanding how DNM2 operates can inform therapeutic strategies for conditions arising from its dysfunction. The development of techniques for producing and characterizing recombinant DNM2 is therefore essential, enabling a deeper understanding of its role in cellular processes and paving the way for potential interventions in DNM2-related diseases.
