Analytical Data
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Gene name
GTF2H1
- Application
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Alternative Names
GTF2-H1; BTF2; TFIIH; BTF2 p62; Basic transcription factor 2 62 kDa subunit; TFIIH basal transcription factor complex p62 subunit; General Transcription Factor IIH, Polypeptide 1
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P32780
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Expression Region
Thr84~Met335
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Molecular Weight
34kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GTF2H1, also known as General Transcription Factor IIH subunit 1, is a pivotal protein involved in the transcription regulation and DNA repair processes in eukaryotic cells. As a core component of the transcription factor IIH (TFIIH) complex, GTF2H1 plays a crucial role in the unwinding of DNA, which is necessary for RNA polymerase II to initiate transcription. Additionally, it participates in nucleotide excision repair, a mechanism for correcting DNA damage caused by environmental factors, such as UV radiation. Mutations or dysregulation of GTF2H1 are associated with various disorders, including xeroderma pigmentosum and Cockayne syndrome, underscoring its importance in maintaining genomic stability. The study and recombinant production of GTF2H1 are essential for elucidating its biochemical properties and functional mechanisms, as well as for developing potential therapeutic strategies for diseases linked to its dysfunction. Understanding the structure and interactions of GTF2H1 may provide insights into the broader context of transcription regulation and DNA repair, facilitating advancements in molecular biology and medicine. Recombination techniques enable the production of this protein in controlled laboratory settings, allowing researchers to investigate its role in cellular processes and assess its potential as a target for drug development. Overall, the exploration of GTF2H1 and its recombinant forms holds significant promise for advancing our comprehension of genetic regulation and the molecular underpinnings of related diseases.
