Analytical Data
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Gene name
CRBN-DDB1
- Application
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Alternative Names
Protein cereblon; CRBN; AD-006; DNA damage-binding protein 1; DDB p127 subunit; XAP1; UV-damaged DNA-binding factor; HBV X-associated protein 1 (XAP-1); Damage-specific DNA-binding protein 1; DDBa
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-Avi
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96SW2-1 (M1-L442) &Q16531-1
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Expression Region
Q96SW2-1 (M1-L442) &Q16531-1 (M1-H1140)
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Molecular Weight
55.3 KDa&126.9 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The CRBN-DDB1 complex is crucial in the context of protein homeostasis and cellular responses to stress, particularly within the realm of ubiquitin-proteasome-mediated degradation pathways. CRBN (Cereblon) is a substrate receptor in the E3 ubiquitin ligase complex, which plays a vital role in targeting specific proteins for degradation by tagging them with ubiquitin. DDB1 (Damaged DNA Binding protein 1) serves as a necessary scaffold in this complex, enhancing the recruitment of CRBN and its substrates. Research on the CRBN-DDB1 complex has gained attention primarily due to its involvement in various biological processes, including cell differentiation and immune response regulation. Moreover, the binding of certain drugs, such as immunomodulatory imide drugs (IMiDs), to CRBN has been linked to therapeutic benefits in treating multiple myeloma. Understanding the structural and functional dynamics of the CRBN-DDB1 complex is essential for unraveling the molecular mechanisms underlying its actions, as well as for identifying potential therapeutic targets in diseases where dysregulated protein degradation plays a role. Thus, recombinant protein studies focusing on CRBN and DDB1 are pivotal for advancing our knowledge of ubiquitin-mediated signaling pathways, and therapeutic innovations in cancer and other diseases associated with protein misfolding and degradation dysregulation.
