Analytical Data
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基因名
SF3B3
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简介
SF3B3 protein is a key splicing factor in the SF3B complex and plays a central role in pre-mRNA splicing. It is essential for "A" complex assembly and stabilizes U2 snRNP binding to branch point sequences. SF3B3 Protein, Human (sf9, His, Strep) is the recombinant human-derived SF3B3 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.
- Application
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别名
SF3B3; Splicing factor 3B subunit 3; Pre-mRNA-splicing factor SF3b 130 kDa subunit; SF3b130; STAF130; Spliceosome-associated protein 130; SAP 130
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种属
Human
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表达系统
Baculovirus
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标签
N-8*His;N-StrepⅡ
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q15393
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表达区间
F2-F1217
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SF3B3 is a critical component of the spliceosomal complex, which plays a pivotal role in the pre-mRNA splicing process that is essential for the maturation of eukaryotic mRNA. The protein is part of the SF3B complex, which is involved in the recognition and selection of splice sites during the splicing of pre-mRNA. Due to its essential role in gene expression regulation, SF3B3 has garnered significant attention in the context of various diseases, particularly cancer, where aberrant splicing is often observed. Research has shown that mutations and alterations in the splicing machinery, including components like SF3B3, can lead to the production of oncoproteins or the loss of tumor suppressor isoforms, thereby contributing to tumorigenesis. Additionally, SF3B3 has been implicated in the resistance mechanisms of certain cancer treatments, highlighting the necessity for a deeper understanding of its function and interactions. Moreover, studies on the recombinant form of SF3B3 can provide insights into its structural and functional properties, enabling the development of targeted therapies that modulate splicing processes. Overall, the comprehensive study of SF3B3, including its recombinant expression and characterization, is crucial for elucidating its role in cellular mechanisms and its potential as a therapeutic target in cancer and other splicing-related disorders.
