Analytical Data
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Gene name
ZNRF3
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简介
ZNRF3, as an E3 ubiquitin protein ligase, plays a crucial role as a negative regulator in the Wnt signaling pathway. It does this by mediating the ubiquitination and subsequent degradation of components within the Wnt receptor complex, namely Frizzled and LRP6. ZNRF3 Protein, Human (HEK293, His) is the recombinant human-derived ZNRF3 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Alternative Names
ZNRF3; Zinc; RING finger protein 3; RING-type E3 ubiquitin transferase ZNRF3; RING finger protein 203; KIAA1133; RNF203
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Species
Human
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9ULT6-1
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Expression Region
K56-M219
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Protein Length
Extracellular Domain
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Molecular Weight
21 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ZNRF3 (Zinc RING Finger Protein 3) is a member of the RING finger protein family, known for its role in regulating cellular processes including cell proliferation and differentiation. Emerging research highlights ZNRF3 as a critical regulator of the Wnt signaling pathway, a key player in development and stem cell maintenance. Dysfunctional Wnt signaling is implicated in various diseases, including cancer, making ZNRF3 an attractive target for therapeutic intervention. The protein is believed to act as an E3 ubiquitin ligase, promoting the degradation of Wnt receptors, thereby inhibiting the pathway's activation. Understanding ZNRF3 at the molecular level, particularly through the development of recombinant protein for functional studies, is essential for elucidating its precise mechanism of action and potential as a drug target. Researchers utilize recombinant ZNRF3 to study its interactions, post-translational modifications, and effects on Wnt signaling, which could reveal new insights into its role in cancer biology and other Wnt-related diseases. As interest in targeted therapies grows, ZNRF3 represents a promising candidate for the development of novel treatment strategies aimed at restoring normal Wnt signaling in pathological conditions. Thus, the investigation of ZNRF3 and its recombinant forms is a significant step towards understanding its contributions to cellular homeostasis and its potential for therapeutic applications.
