Analytical Data
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Gene name
BK
- Application
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Species
Human
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Source
HEK293
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
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Expression Region
Synthetic Peptide
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Molecular Weight
>800KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
The study of BK (Big Potassium) channel proteins has garnered significant interest due to their crucial role in various physiological processes and their association with numerous pathophysiological conditions. These large, voltage- and calcium-gated potassium channels, encoded by the KCNMA1 gene, are primarily found in a variety of tissues, including the brain, heart, and smooth muscle cells. BK channels are essential for maintaining cellular excitability, regulating neurotransmitter release, and controlling muscle contraction. Their unique activation mechanism, which integrates both voltage and intracellular calcium levels, allows them to respond to varying physiological stimuli dynamically. Research has shown that dysregulation of BK channel function is implicated in several disorders, such as hypertension, epilepsy, and certain types of cancer. This has spurred the exploration of BK channel modulators as therapeutic agents. The effort to understand the structure-function relationship of BK proteins has also advanced significantly, aided by techniques such as X-ray crystallography and cryo-electron microscopy, revealing the intricate details of their molecular architecture. As a result, the ongoing research into BK channels not only aims to elucidate their biological roles but also to develop targeted therapies that can effectively modulate their activity in disease states, highlighting their potential as significant pharmacological targets.
