Analytical Data
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Gene name
EDEM2
- Application
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Alternative Names
ER degradation-enhancing alpha-mannosidase-like protein 2; C20orf31; C20orf49
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Species
Human
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Source
HEK293
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Tag
C-10*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
AAH01371.1
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Expression Region
A22-K492
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AA Sequence
APGPDGSAPDPAHYRERVKAMFYHAYDSYLENAFPFDELRPLTCDGHDTWGSFSLTLIDALDTLLILGNVSEFQRVVEVLQDSVDFDIDVNASVFETNIRVVGGLLSAHLLSKKAGVEVEAGWPCSGPLLRMAEEAARKLLPAFQTPTGMPYGTVNLLHGVNPGETPVTCTAGIGTFIVEFATLSSLTGDPVFEDVARVALMRLWESRSDIGLVGNHIDVLTGKWVAQDAGIGAGVDSYFEYLVKGAILLQDKKLMAMFLEYNKAIRNYTRFDDWYLWVQMYKGTVSMPVFQSLEAYWPGLQSLIGDIDNAMRTFLNYYTVWKQFGGLPEFYNIPQGYTVEKREGYPLRPELIESAMYLYRATGDPTLLELGRDAVESIEKISKVECGFATIKDLRDHKLDNRMESFFLAETVKYLYLLFDPTNFIHNNGSTFDTVITPYGECILGAGGYIFNTEAHPIDPAALHCCQRLK
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Protein Length
Partial
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Molecular Weight
50-75 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EDEM2 (ER degradation-enhancing mannosidase-like protein 2) is a crucial component in the endoplasmic reticulum (ER) quality control system, primarily involved in the recognition and processing of misfolded glycoproteins for degradation. Located in the ER, EDEM2 plays a significant role in the lectin-like recognition of glycosylated substrates, promoting their extraction from the ER and facilitating their transport to the proteasome for degradation via the ER-associated degradation (ERAD) pathway. Research on EDEM2 has gained momentum due to its implications in various diseases related to protein misfolding, including neurodegenerative disorders and certain types of cancer. The understanding of EDEM2's function can provide insights into therapeutic strategies aimed at enhancing ERAD efficiency, potentially alleviating the burden of protein aggregation in affected tissues. Moreover, the investigation of EDEM2 and its interactions with other ER quality control components can illuminate the broader mechanisms of cellular stress responses, contributing to the development of biologically-derived treatments for protein misfolding disorders. As the field of protein homeostasis continues to evolve, EDEM2 stands out as a pivotal target for research aimed at understanding the complexities of protein folding, degradation, and associated pathologies.
