Analytical Data
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基因名
GUCY2C/Guanylyl cyclase C
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简介
GUCY2C, a guanylyl cyclase, catalyzes cGMP synthesis from GTP and acts as a receptor for E. coli heat-stable enterotoxin, stimulating cGMP accumulation in expressing mammalian cells. Additionally, endogenous peptides guanylin and uroguanylin activate GUCY2C. This multifaceted role underscores intricate regulatory mechanisms in cellular signaling and GUCY2C's responsiveness to various stimuli, as confirmed by studies. GUCY2C/Guanylyl cyclase C Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived GUCY2C/Guanylyl cyclase C protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
- Application
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生物活性
1. Immobilized Anti-GUCY2C Antibody at 1 μg/mL (100μL/Well) on the plate. Dose response curve for Biotinylated Human GUCY2C with the EC50 of 24.1-33.6 ng/mL determined by ELISA. 2. Loaded Indusatumab (HY-P99232) on AHC2 biosensor, can bind GUCY2C/Guanylyl cyclase C Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 2.157E-08 M as determined in BLI assay. Loaded Indusatumab (HY-P99232) on AHC2 biosensor, can bind GUCY2C/Guanylyl cyclase C Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 2.157E-08 M as determined in BLI assay.
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别名
Guanylyl cyclase C; GC-C; STAR; GUCY2C; GUC2C; STA receptor; DIAR6; EC 4.6.1; GCC; GUC2CEC 4.6.1.2; MUCIL
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种属
Human
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表达系统
HEK293
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标签
C-Avi;C-8*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P25092
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表达区间
S24-Q430
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蛋白长度
Extracellular Domain
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分子量
55-65 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Guanylyl cyclase C (GC-C) is a receptor guanylyl cyclase that plays a crucial role in the regulation of fluid homeostasis, digestive function, and cell signaling. It is primarily activated by two ligands: guanylin and uroguanylin, which are secreted by intestinal cells. Upon activation, GC-C catalyzes the conversion of GTP to cyclic GMP (cGMP), a second messenger that triggers various physiological responses. Dysregulation of GC-C signaling has been implicated in several gastrointestinal disorders, including diarrhea and inflammatory bowel disease, making it a potential therapeutic target. Research on recombinant forms of GC-C, such as GUCY2C, has advanced our understanding of its structure and function, particularly in the context of ligand binding and downstream signaling pathways. Utilizing recombinant proteins allows for detailed biochemical studies, elucidating the molecular mechanisms underlying GC-C activity and its interactions with various signaling molecules. Understanding these processes has significant implications for developing novel treatments for related diseases, highlighting the importance of GC-C research in biomedicine.
