Cat: IPD-X33654

Recombinant Rat HRP-Labeled Alkaline Phosphatase/ALPL Protein,His & SUMO

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Analytical Data

  • 基因名

    HRP-Labeled Alkaline Phosphatase/ALPL

  • Application

    SPRMSTBLIITCELISA细胞实验药物筛选

  • 别名

    Alkaline phosphatase liver/bone/kidney isozyme

  • 种属

    Rat

  • 表达系统

    E. coli

  • 标签

    N- His-SUMO

  • 纯度

    Greater than 90% as determined by SDS-PAGE.

  • 蛋白编号

    P08289

  • 表达区间

    18-501aa

  • 分子量

    69.5 kDa

  • 内毒素

    < 1.0 EU per μg protein as determined by the LAL method.

  • 性状

    Freeze-dried powder

  • 缓冲液

    PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

  • 复溶方法

    Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

  • 个性化定制

    点位突变 标签定制 buffer定制 全长蛋白定制

  • 稳定性测试

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

  • 保存条件 & 期限

    Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

  • 运输条件

    In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.

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Protein Description

Recombinant Alkaline Phosphatase (ALPL) labeled with horseradish peroxidase (HRP) represents a significant advancement in the field of biochemistry and molecular biology. ALPL is an essential enzyme involved in various physiological processes, including bone mineralization and the regulation of phosphate levels. The study of ALPL has gained attention due to its implications in bone-related diseases and disorders, such as hypophosphatasia and osteoporosis. The fusion with HRP allows for enhanced detection and quantification capabilities in experimental assays, facilitating studies in enzymology, diagnostics, and therapeutic applications. Using HRP labeling not only improves the stability and activity of ALPL but also enables sensitive colorimetric or chemiluminescent detection methods, making it a valuable tool in research and clinical settings. The characterization and optimization of HRP-labeled ALPL have the potential to advance our understanding of its biological functions and lead to new strategies in the treatment of metabolic bone diseases. Furthermore, this recombinant protein can be utilized in various applications, including enzyme-linked immunosorbent assays (ELISAs), where accurate measurement of enzyme activity is crucial for diagnosing health conditions. Thus, the exploration of HRP-labeled ALPL is pivotal for both fundamental research and translational medicine.

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IPODIX North America (HQ)
Proteintech Group, Inc
5500 Pearl Street, Suite 400
Rosemont, IL 60018, USA
1-888-478-4522
proteintech@ptglab.com
IPODIX North America (HQ)
Proteintech Group, Inc
5500 Pearl Street, Suite 400
Rosemont, IL 60018, USA
1-888-478-4522
proteintech@ptglab.com
IPODIX North America (HQ)
Proteintech Group, Inc
5500 Pearl Street, Suite 400
Rosemont, IL 60018, USA
1-888-478-4522
proteintech@ptglab.com
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