Recombinant Human BPI Protein (HEK293)，His

BPI

BPI Protein, Human (HEK293, His), a member of a genomically conserved lipid-interactive protein family, belongs to a diverse group of polypeptides with antimicrobial activity that are thought to participate in antimicrobial host defence[1][2][3].

SPRMSTBLIITCELISACELL ASSAYDRUG SCREENING

Measured by its ability to inhibit LPS-induced IL-8 secretion by THP-1 human acute monocytic leukemia cell. The ED50 for this effect is 3.082 ng/mL in the present of 2 μg/mL LPS, corresponding to a specific activity is 3.245×105 U/mg. Measured by its ability to inhibit LPS-induced IL-8 secretion by THP-1 human acute monocytic leukemia cell. The ED50 for this effect is 3.082 ng/mL in the present of 2 μg/mL LPS, corresponding to a specific activity is 3.245×105 U/mg.

rHuBactericidal Permeability-Increasing Protein, His; Bactericidal permeability-increasing protein; BPI; CAP57

Human

HEK293

C-6*His

Greater than 90% as determined by SDS-PAGE.

AAH40955.1

V32-K487

VNPGVVVRISQKGLDYASQQGTAALQKELKRIKIPDYSDSFKIKHLGKGHYSFYSMDIREFQLPSSQISMVPNVGLKFSISNANIKISGKWKAQKRFLKMSGNFDLSIEGMSISADLKLGSNPTSGKPTITCSSCSSHINSVHVHISKSKVGWLIQLFHKKIESALRNKMNSQVCEKVTNSVSSKLQPYFQTLPVMTKIDSVAGINYGLVAPPATTAETLDVQMKGEFYSENHHNPPPFAPPVMEFPAAHDRMVYLGLSDYFFNTAGLVYQEAGVLKMTLRDDMIPKESKFRLTTKFFGTFLPEVAKKFPNMKIQIHVSASTPPHLSVQPTGLTFYPAVDVQAFAVLPNSSLASLFLIGMHTTGSMEVSAESNRLVGELKLDRLLLELKHSNIGPFPVELLQDIMNYIVPILVLPRVNEKLQKGFPLPTPARVQLYNVVLQPHQNFLLFGADVVYKHHHHHH

Partial

50-60 kDa

< 1.0 EU per μg protein as determined by the LAL method.

Freeze-dried powder

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.

Site-directed mutagenesis Custom tag design Custom buffer formulation Custom full-length protein production

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.

Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.