Analytical Data
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Gene name
ECSIT
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简介
ECSIT protein is an adapter protein in Toll-like receptors, IL-1, and antiviral signaling that crucially activates NF-kappa-B by complexing with TRAF6 and TAK1/MAP3K7, leading to TAK1/MAP3K7 activation and subsequent IKK activation. Ubiquitinated ECSIT interacts with RELA and NFKB1 and translocates to induce NF-kappa-B-dependent gene expression. ECSIT Protein, Human (His) is the recombinant human-derived ECSIT protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial; Protein SITPEC; ECSIT
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q9BQ95-1
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Expression Region
E246-S431
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AA Sequence
QVPLPKDSTGAADPPQPHIVGIQSPDQQAALARHNPARPVFVEGPFSLWLRNKCVYYHILRADLLPPEEREVEETPEEWNLYYPMQLDLEYVRSGWDNYEFDINEVEEGPVFAMCMAGAHDQATMAKWIQGLQETNPTLAQIPVVFRLAGSTRELQTSSAGLEEPPLPEDHQEEDDNLQRQQQGQS
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Protein Length
Partial
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Molecular Weight
25 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EEF1E1, or elongation factor 1 epsilon 1, is a crucial protein involved in the process of translation in eukaryotic cells. This protein plays a significant role in the elongation phase of protein synthesis by facilitating the delivery of aminoacyl-tRNAs to the ribosome. Recent research has highlighted the multifunctional nature of EEF1E1 beyond its primary role in translation, suggesting its involvement in various cellular processes, including cell signaling, stress response, and apoptosis. The study of recombinant EEF1E1 protein has gained attention due to its potential implications in understanding diseases, such as cancer and neurodegenerative disorders, where protein synthesis and cellular stress responses are often dysregulated. By producing and characterizing recombinant EEF1E1, researchers aim to explore its structural properties and interactions with other cellular components, providing insights into its functional mechanisms. Additionally, recombinant EEF1E1 can serve as a valuable tool in investigating the translation machinery and its regulation, paving the way for novel therapeutic strategies targeting translation-related pathways in pathological conditions. The growing body of research on EEF1E1 underscores its significance in cellular biology and medicine, highlighting the need for continued exploration of this protein in both basic and applied research contexts.
