Analytical Data
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Gene name
Arylsulfatase A/ARSA
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简介
Arylsulfatase A (ARSA) possesses arylsulfatase and calcium ion binding activities. It plays a critical role in sperm-zona pellucida binding and is located in the plasma membrane. ARSA is expressed in the brain and genitourinary system, reflecting its involvement in various processes. It is associated with metachromatic leukodystrophy and broadly expressed across tissues, highlighting its significance in diverse contexts. Arylsulfatase A/ARSA Protein, Mouse (HEK293, His) is the recombinant mouse-derived Arylsulfatase A/ARSA protein, expressed by HEK293 , with C-10*His labeled tag.
- Application
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Alternative Names
ARSA; Arylsulfatase A; ASA; MLD
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Species
Mouse
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Source
HEK293
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Tag
C-10*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P50428
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Expression Region
S18-S506
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AA Sequence
SPPNILLIFADDLGYGDLGSYGHPSSTTPNLDQLAEGGLRFTDFYVPVSLCTPSRAALLTGRLPVRSGMYPGVLGPSSQGGLPLEEVTLAEVLAARGYLTGMAGKWHLGVGPEGAFLPPHQGFHRFLGIPYSHDQGPCQNLTCFPPDIPCKGGCDQGLVPIPLLANLTVEAQPPWLPGLEARYVSFSRDLMADAQRQGRPFFLYYASHHTHYPQFSGQSFTKRSGRGPFGDSLMELDGAVGALMTTVGDLGLLEETLVIFTADNGPELMRMSNGGCSGLLRCGKGTTFEGGVREPALVYWPGHITPGVTHELASSLDLLPTLAALTGAPLPNVTLDGVDISPLLLGTGKSPRKSVFFYPPYPDEIHGVFAVRNGKYKAHFFTQGSAHSDTTSDPACHAANRLTAHEPPLLYDLSQDPGENYNVLESIEGVSPEALQALKHIQLLKAQYDAAMTFGPSQIAKGEDPALQICCQPSCTPHPVCCHCPGSQS
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Protein Length
Full Length of Mature Protein
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Molecular Weight
65 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Arylsulfatase A (ARSA) is a crucial enzyme involved in the catabolism of sulfated glycosaminoglycans and is primarily expressed in the lysosomes. Deficiency of ARSA leads to a rare genetic disorder known as Metachromatic Leukodystrophy (MLD), characterized by the accumulation of sulfated lipids, demyelination, and neurodegeneration. Given the severe neurological implications of ARSA deficiency, there has been a significant focus on developing therapeutic strategies, including enzyme replacement therapy (ERT). The recombinant production of ARSA has become a central aspect of these efforts. Understanding the structure-function relationships of ARSA, optimizing its production in various expression systems, and enhancing its stability and delivery are vital for developing effective treatment options for MLD patients. Recent advancements in recombinant DNA technology have enabled the generation of functional and commercially viable ARSA proteins, paving the way for clinical applications. Furthermore, the exploration of potential gene therapy approaches and small molecule pharmacological chaperones is also underway to correct the underlying genetic defects associated with ARSA deficiency. As research continues to unfold, the dual focus on the biochemical characterization of ARSA and the evaluation of innovative therapeutic modalities represents a promising frontier in the battle against MLD and related lysosomal storage disorders.
