Analytical Data
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Gene name
PRKN
- Application
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Alternative Names
AR-JP; LPRS2; PDJ; PRKN; Parkin; Autosomal Recessive,Juvenile; E3 ubiquitin-protein ligase parkin
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60260
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Expression Region
Ile229~Val465
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Molecular Weight
30kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PRKN, or Parkin, is an E3 ubiquitin ligase that plays a critical role in the ubiquitin-proteasome system, which is essential for protein degradation and cellular homeostasis. Mutations in the PRKN gene have been linked to autosomal recessive juvenile Parkinsonism, making it a significant focus of research in neurodegenerative diseases. Parkin's involvement in mitochondrial quality control, particularly its role in promoting the degradation of defective mitochondria through a process called mitophagy, highlights its importance in maintaining cellular function and preventing neurodegeneration. As researchers delve deeper into the mechanisms underlying PRKN activity, they aim to uncover potential therapeutic strategies for Parkinson's disease and other related disorders. The exploration of PRKN's structure and function, along with its interactions with other cellular proteins, provides valuable insights into the pathological processes of neurodegeneration, offering possibilities for targeted interventions that could mitigate disease progression and improve patient outcomes. Given the increasing prevalence of Parkinson's disease in aging populations, understanding PRKN's role in cellular pathology remains a crucial area of investigation, with implications for developing innovative treatments in the field of neurology.
