Analytical Data
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基因名
Niemann Pick C1/NPC1
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简介
The Niemann Pick C1/NPC1 protein is an intracellular cholesterol transporter that cooperates with NPC2 to promote cholesterol efflux from the endosomal/lysosomal compartment. NPC2 transfers unesterified cholesterol to the N-terminal domain of NPC1, where the cholesterol is bound in a hydrophobic pocket. Niemann Pick C1/NPC1 Protein, Human (HEK293, His) is the recombinant human-derived Niemann Pick C1/NPC1 protein, expressed by HEK293 , with N-6*His labeled tag.
- Application
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别名
NPC intracellular cholesterol transporter 1; Niemann-Pick C1 protein; NPC1
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种属
Human
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表达系统
HEK293
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标签
N-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
O15118-1
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表达区间
R372-F622
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氨基酸序列
RVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGADVPFGPPLDIQILHQVLDLQIAIENITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDTEKLQRAQAWEKEFINFVKNYKNPNLTISFTAERSIEDELNRESDSDVF
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蛋白长度
Lumenal Domain
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分子量
38-55 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Niemann-Pick disease type C1 (NPC1) is a inherited lysosomal lipid storage disorder characterized by the impaired intracellular transport of cholesterol and other lipids, leading to their accumulation in various tissues, particularly the brain and liver. Mutations in the NPC1 gene are responsible for the majority of NPC cases, and the NPC1 protein plays a crucial role in the mobilization of cholesterol from late endosomes and lysosomes to other cellular compartments. Research into NPC1 protein has garnered significant attention due to its implications in neurodegenerative diseases and the aging process. Understanding the structure and function of the NPC1 protein is vital for developing therapeutic strategies for NPC and related disorders. Recent studies utilizing recombinant NPC1 proteins have provided insights into the protein's conformational dynamics, lipid binding properties, and its interactions within the cellular environment. By characterizing recombinant NPC1, researchers aim to elucidate the molecular mechanisms underlying cholesterol trafficking and explore potential avenues for pharmacological intervention, thereby addressing the unmet medical need for effective treatments for NPC and helping mitigate the disease's impact on affected individuals and their families.
