Analytical Data
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Gene name
IL-27 beta
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简介
The IL-27 beta/EBI3 protein binds to IL27 to form IL-27 interleukin, which is critical in innate immunity. IL-27 has both pro- and anti-inflammatory properties, regulating T helper cell development, inhibiting T cell proliferation, stimulating cytotoxic T cell activity, inducing B cell isotype switching, and affecting innate immune cells. Animal-Free IL-27 beta/EBI3 Protein, Human (His) is the recombinant human-derived animal-FreeIL-27 beta/EBI3 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.
- Application
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Alternative Names
Interleukin-27 subunit alpha; IL-27-A; Interleukin-27 subunit beta; IL-27B; Epstein-Barr virus-in_x0002_duced gene 3 protein; EBV-induced gene 3 protein; EBI3; p28; Interleukin-30; IL-30
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Species
Human
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Source
E. coli
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Tag
C-6*His
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Purity
> 90% as determined by SDS-PAGE.
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Uniprot
Q14213
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Expression Region
R21-K229
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AA Sequence
MRKGPPAALTLPRVQCRASRYPIAVDCSWTLPPAPNSTSPVSFIATYRLGMAARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVTAVHPWGSSSSFVPFITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYKRQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLPATATMSLGK
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Protein Length
Full Length of Mature Protein
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Molecular Weight
25 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
IL-27 is a member of the interleukin-6/12 cytokine family, primarily produced by antigen-presenting cells, and plays a crucial role in regulating immune responses. Its structure consists of two subunits, p28 and Ebi3, which together form the biologically active heterodimer. The beta subunit of IL-27, specifically the Ebi3 component, has garnered significant interest in immunological research due to its involvement in promoting T helper 1 (Th1) cell differentiation, inhibiting Th2 cell responses, and modulating the activity of regulatory T cells. Dysregulation of IL-27 signaling has been associated with various inflammatory and autoimmune diseases, including multiple sclerosis, rheumatoid arthritis, and cancer. The recombinant production of IL-27 beta (Ebi3) protein allows for detailed studies into its biological functions and potential therapeutic applications. Researchers are exploring how modulating IL-27 signaling could provide new avenues for treating immune-related conditions by either enhancing or inhibiting its activity. Given its complex role in both promoting and regulating immune responses, understanding the mechanisms through which IL-27 beta operates can contribute to the development of novel immunotherapies and enhance our grasp of the immune system’s intricate dynamics.
