Analytical Data
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基因名
IL-27 beta
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简介
The IL-27 beta/EBI3 protein binds to IL27 to form IL-27 interleukin, which is critical in innate immunity. IL-27 has both pro- and anti-inflammatory properties, regulating T helper cell development, inhibiting T cell proliferation, stimulating cytotoxic T cell activity, inducing B cell isotype switching, and affecting innate immune cells. Animal-Free IL-27 beta/EBI3 Protein, Human (His) is the recombinant human-derived animal-FreeIL-27 beta/EBI3 protein, expressed by E. coli , with C-His labeled tag. This product is for cell culture use only.
- Application
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别名
Interleukin-27 subunit alpha; IL-27-A; Interleukin-27 subunit beta; IL-27B; Epstein-Barr virus-in_x0002_duced gene 3 protein; EBV-induced gene 3 protein; EBI3; p28; Interleukin-30; IL-30
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种属
Human
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表达系统
E. coli
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标签
C-6*His
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纯度
> 90% as determined by SDS-PAGE.
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蛋白编号
Q14213
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表达区间
R21-K229
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氨基酸序列
MRKGPPAALTLPRVQCRASRYPIAVDCSWTLPPAPNSTSPVSFIATYRLGMAARGHSWPCLQQTPTSTSCTITDVQLFSMAPYVLNVTAVHPWGSSSSFVPFITEHIIKPDPPEGVRLSPLAERQLQVQWEPPGSWPFPEIFSLKYWIRYKRQGAARFHRVGPIEATSFILRAVRPRARYYVQVAAQDLTDYGELSDWSLPATATMSLGK
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蛋白长度
Full Length of Mature Protein
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分子量
25 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
IL-27 is a member of the interleukin-6/12 cytokine family, primarily produced by antigen-presenting cells, and plays a crucial role in regulating immune responses. Its structure consists of two subunits, p28 and Ebi3, which together form the biologically active heterodimer. The beta subunit of IL-27, specifically the Ebi3 component, has garnered significant interest in immunological research due to its involvement in promoting T helper 1 (Th1) cell differentiation, inhibiting Th2 cell responses, and modulating the activity of regulatory T cells. Dysregulation of IL-27 signaling has been associated with various inflammatory and autoimmune diseases, including multiple sclerosis, rheumatoid arthritis, and cancer. The recombinant production of IL-27 beta (Ebi3) protein allows for detailed studies into its biological functions and potential therapeutic applications. Researchers are exploring how modulating IL-27 signaling could provide new avenues for treating immune-related conditions by either enhancing or inhibiting its activity. Given its complex role in both promoting and regulating immune responses, understanding the mechanisms through which IL-27 beta operates can contribute to the development of novel immunotherapies and enhance our grasp of the immune system’s intricate dynamics.
