Analytical Data
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Gene name
TLK2
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简介
TLK2 is an important serine/threonine protein kinase with complex effects on chromatin assembly, DNA replication, transcription, repair, and chromosome segregation. It plays multiple roles by phosphorylating and stabilizing chromatin assembly factors such as ASF1A and ASF1B, preventing proteasome-mediated degradation. TLK2 Protein, Human (749a.a, sf9, His, GST) is the recombinant human-derived TLK2, expressed by Sf9 insect cells , with His, GST labeled tag. ,
- Application
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Alternative Names
TLK2; tousled-like kinase 2; serine/threonine-protein kinase tousled-like 2; MGC44450; PKU ALPHA; serine/threonine kinase; PKU-ALPHA
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Species
Human
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Source
Baculovirus
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Tag
N-8*His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q86UE8-1
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Expression Region
M2-S750
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Protein Length
Partial
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TLK2 (Tousled-like kinase 2) is a serine/threonine kinase that plays a crucial role in various cellular processes, including DNA damage response, cell cycle regulation, and chromatin remodeling. Its involvement in maintaining genomic stability has raised significant interest in the scientific community, particularly relating to cancer research, where aberrant kinase activity often contributes to tumorigenesis. TLK2 is known to interact with several substrates involved in the repair of DNA double-strand breaks and the regulation of gene expression, suggesting its vital role in the cellular response to stressors. The study of TLK2 recombinant protein offers insights into its precise biochemical mechanisms, potential as a therapeutic target, and its interactions within the cellular environment. Furthermore, understanding the structure-function relationship of TLK2 through recombinant protein studies can facilitate the design of small-molecule inhibitors, aiming to modulate its activity in pathological conditions. Advances in recombinant DNA technology have enabled the production of TLK2 in a purified form, providing researchers with a valuable tool for elucidating its functional properties and exploring its role in cancer progression and other diseases. This research not only contributes to the fundamental knowledge of kinase biology but also paves the way for novel therapeutic strategies aimed at diseases associated with TLK2 dysregulation.
