Analytical Data
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基因名
CRELD1
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简介
CRELD1 Protein, acting as a protein disulfide isomerase, aids in the correct folding and assembly of proteins, crucial for cellular homeostasis. It also contributes to the proper localization of acetylcholine receptors (AChRs) to the plasma membrane, emphasizing its significance in maintaining protein structure and membrane receptor distribution. CRELD1 Protein, Human (HEK293, His) is the recombinant human-derived CRELD1 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.5052 μg/mL, corresponding to a specific activity is 1979.022 units/mg. Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED50 for this effect is 0.5052 μg/mL, corresponding to a specific activity is 1979.022 units/mg.
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别名
Protein disulfide isomerase CRELD1; Cysteine-rich with EGF-like domain protein 1; CIRRIN
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q96HD1-1
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表达区间
Q30-E362
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氨基酸序列
QPSPPPQSSPPPQPHPCHTCRGLVDSFNKGLERTIRDNFGGGNTAWEEENLSKYKDSETRLVEVLEGVCSKSDFECHRLLELSEELVESWWFHKQQEAPDLFQWLCSDSLKLCCPAGTFGPSCLPCPGGTERPCGGYGQCEGEGTRGGSGHCDCQAGYGGEACGQCGLGYFEAERNASHLVCSACFGPCARCSGPEESNCLQCKKGWALHHLKCVDIDECGTEGANCGADQFCVNTEGSYECRDCAKACLGCMGAGPGRCKKCSPGYQQVGSKCLDVDECETEVCPGENKQCENTEGGYRCICAEGYKQMEGICVKEQIPESAGFFSEMTEDE
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蛋白长度
Extracellular Domain
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分子量
45-50 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CRELD1 (Cysteine-Rich Endoplasmic Reticulum Protein 1) is a critical protein that plays a significant role in the development of various tissues, particularly in cardiac and neural systems. Research indicates that mutations in the CRELD1 gene are associated with congenital heart defects and other developmental disorders, highlighting its importance in embryonic development and tissue morphogenesis. The protein is localized in the endoplasmic reticulum and is thought to be involved in protein folding and assembly, suggesting its essential function in maintaining cellular homeostasis. Given its role in congenital malformations and its potential as a therapeutic target, the study of CRELD1 recombinant proteins has gained traction as a way to better understand its functional mechanisms and interactions. The production and characterization of CRELD1 recombinant proteins allow researchers to explore the effects of specific mutations, delineate the pathways it influences, and identify potential pharmacological interventions. Through techniques such as in vitro assays and structural analysis, scientists aim to unravel the complex roles of CRELD1 in cellular processes and its implications in disease, thus paving the way for future advancements in treating conditions linked to its dysregulation. The ongoing research into CRELD1 not only enhances our understanding of developmental biology but also holds promise for the development of targeted therapies to address congenital defects and related disorders.
