Analytical Data
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Gene name
TPM1
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简介
TPM1 encodes tropomyosin, a key actin-binding component in muscle contraction and cytoskeletal structure. It contains α-helical chains that form a coiled-coil structure that stabilizes actin filaments. TPM1 Protein, Human (His) is the recombinant human-derived TPM1 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Tropomyosin alpha-1 chain; Tropomyosin-1; TPM1; C15orf13; TMSA
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P09493-10
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Expression Region
M1-M284
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AA Sequence
MDAIKKKMQMLKLDKENALDRAEQAEADKKAAEDRSKQLEDELVSLQKKLKGTEDELDKYSEALKDAQEKLELAEKKATDAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGQVRQLEEQLRIMDQTLKALMAAEDKYSQKEDRYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDELYAQKLKYKAISEELDHALNDMTSM
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Protein Length
Full Length of Isoform-10
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Molecular Weight
35 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TPM1 (Tropomyosin 1) is a key protein that plays a crucial role in the regulation of muscle contraction and cytoskeletal organization. It is a part of the tropomyosin family, which is essential for maintaining the stability and functionality of actin filaments in various cell types, including muscle cells, fibroblasts, and neurons. The study of recombinant TPM1 proteins is significant due to their potential implications in understanding various muscle disorders and cardiac diseases linked to mutations in the TPM1 gene. These mutations can lead to disruptions in muscle function, resulting in conditions such as familial hypertrophic cardiomyopathy and skeletal myopathies. By producing recombinant TPM1 proteins, researchers can explore the biophysical properties, binding interactions, and functional dynamics of this protein, providing insights into how alterations in TPM1 affect muscle mechanics at a molecular level. Furthermore, recombinant TPM1 can be utilized in drug screening and the development of therapeutic proteins aimed at rescuing function in affected patients, making it a valuable target in both fundamental research and clinical applications. The increasing advances in protein expression technology, coupled with high-throughput screening techniques, have paved the way for more in-depth studies into the structure-function relationships of TPM1, thus enhancing our understanding of its role in health and disease.
