Analytical Data
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基因名
CDK9-CCNT2
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简介
The CDK9 protein plays a central role in transcriptional regulation, promoting the transition from ineffective to efficient elongation by phosphorylating POLR2A, SUPT5H, and RDBP. As part of the CDK9/cyclin-T complex, it participates in phosphorylation events affecting EP300, MYOD1, RPB1/POLR2A, AR, DSIF, and NELFE. CDK9-CCNT2 Protein, Human (sf9, GST, Flag, His) is the recombinant human-derived CDK9-CCNT2, expressed by Sf9 insect cells, with N-His, N-GST, N-Flag labeled tag.
- Application
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别名
CDK9; CCNK; Cyclin-dependent kinase 9; C-2K; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; Serine/threonine-protein kinase PITALRE; Tat-associated kinase complex catalytic subunit; Cyclin-K
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种属
Human
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表达系统
Baculovirus
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标签
N-His;N-GST;N-Flag
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P50750-1 (M1-F372)&A0A2R9BFR5
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表达区间
P50750-1 (M1-F372)&A0A2R9BFR5 (A2-S663)
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CDK9-CCNT2 is a crucial protein complex that plays a significant role in regulating transcriptional processes by phosphorylating the C-terminal domain of RNA polymerase II (Pol II). The cyclin-dependent kinase 9 (CDK9) is known for its involvement in the positive transcription elongation factor b (P-TEFb) complex, which enhances the transition of Pol II from a paused state to productive elongation during RNA synthesis. CCNT2, a cyclin type transcribed in response to various cellular signals, acts as a regulatory partner to CDK9, modulating its activity and stability. Research into the CDK9-CCNT2 complex has garnered attention due to its implications in various pathophysiological conditions, including cancer and viral infections, where dysregulation of transcriptional machinery can lead to uncontrolled cellular proliferation and survival. The study of recombinant CDK9-CCNT2 proteins enables the exploration of their biochemical properties, functional mechanisms, and interaction networks, providing insights into their roles in transcription regulation. Moreover, understanding the structure-function relationship of this complex could inform the development of novel therapeutic strategies targeting aberrant transcriptional control in diseases. Therefore, the research on CDK9-CCNT2 recombinant proteins is essential for advancing our knowledge of the transcriptional landscape and its implications in health and disease.
