Analytical Data
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基因名
IDI2
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简介
IDI2 protein plays a crucial role in catalyzing the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP), leading to the formation of its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). This enzymatic activity is a key step in the biosynthetic pathway, contributing to the generation of essential building blocks for various cellular processes. IDI2 Protein, Human (His) is the recombinant human-derived IDI2 protein, expressed by E. coli , with N-6*His labeled tag.
- Application
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别名
Isopentenyl-Diphosphate Delta-Isomerase 2; Isopentenyl Pyrophosphate Isomerase 2; IPP Isomerase 2; IPPI2; IDI2
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种属
Human
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表达系统
E. coli
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9BXS1
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表达区间
M1-V227
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氨基酸序列
MSDINLDWVDRRQLQRLEEMLIVVDENDKVIGADTKRNCHLNENIEKGLLHRAFSVVLFNTKNRILIQQRSDTKVTFPGYFTDSCSSHPLYNPAELEEKDAIGVRRAAQRRLQAELGIPGEQISPEDIVFMTIYHHKAKSDRIWGEHEICYLLLVRKNVTLNPDPSETKSILYLSQEELWELLEREARGEVKVTPWLRTIAERFLYRWWPHLDDVTPFVELHKIHRV
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蛋白长度
Full Length
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分子量
28-31 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
IDI2 (Isoprenoid synthase domain-containing protein 2) is an essential enzyme involved in the mevalonate pathway, which plays a crucial role in the biosynthesis of isoprenoids, including cholesterol and various terpenoids. This pathway is pivotal for cellular processes such as membrane fluidity, protein prenylation, and the synthesis of hormones and vitamins. Given the importance of isoprenoids in both basic cellular functions and their implications in human health, researchers have focused on recombinant expression of IDI2 to better understand its structure, function, and potential as a therapeutic target. Studies have indicated that IDI2 has unique properties that differentiate it from its homologs, making it a subject of interest in metabolic engineering and drug design. Moreover, disorders related to the mevalonate pathway, such as Smith-Lemli-Opitz syndrome, highlight the necessity for a deeper understanding of IDI2's role and regulation. Recombinant protein technology allows scientists to produce IDI2 in a controlled manner, paving the way for detailed kinetic and structural analyses that could reveal novel insights into its enzymatic mechanisms and interactions. In summary, research on recombinant IDI2 is crucial for elucidating its biological functions and therapeutic potentials, providing a foundation for future studies aimed at manipulating isoprenoid biosynthesis for medical and biotechnological applications.
