Analytical Data
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Gene name
ACOX1
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简介
The ACOX1 protein is critical in the initial steps of peroxisomal β-oxidation, especially of very long-chain fatty acids. It catalyzes the desaturation of fatty acyl-CoA (such as palmitoyl-CoA) to 2-trans-enoyl-CoA. ACOX1 Protein, Human (sf9, His) is the recombinant human-derived ACOX1 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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Alternative Names
Peroxisomal acyl-coenzyme A oxidase 1; AOX; SCOX; ACOX1
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Species
Human
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Source
Baculovirus
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Tag
N-10*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
AAH08767.1
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Expression Region
M1-L660
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Protein Length
Full Length
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Molecular Weight
60 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ACOX1 (Acyl-CoA oxidase 1) is a key enzyme in the peroxisomal β-oxidation pathway, primarily responsible for the oxidation of very long-chain fatty acids. Mutations in the ACOX1 gene have been linked to severe metabolic disorders, including peroxisomal biogenesis disorders and other lipid metabolism abnormalities. The functional study of ACOX1 is essential for understanding the mechanisms underlying these diseases and developing potential therapeutic strategies. Recombinant ACOX1 protein has emerged as a valuable tool for biochemical and structural studies, enabling researchers to investigate its enzymatic activity, substrate specificity, and regulatory mechanisms in vitro. Furthermore, elucidating the structure-function relationship of ACOX1 can pave the way for drug design and the development of enzyme replacement therapies. Recent advancements in protein expression systems have facilitated the production of high yields of active ACOX1, providing insights into its role in fatty acid metabolism and its implications in health and disease. As research progresses, ACOX1 continues to be a focal point for understanding metabolic regulation and peroxisomal function.
