Analytical Data
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基因名
CDK2-CCNE2
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简介
CDK2 is an important serine/threonine protein kinase that plays a critical regulatory role in the cell cycle, especially during meiosis. It phosphorylates various substrates (CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2) and coordinates centrosome duplication and initiation of DNA synthesis at the G1-S transition. CDK2-CCNE2 Protein, Human (sf9, GST, Flag, His) is the recombinant human-derived CDK2-CCNE2, expressed by Sf9 insect cells, with N-His, N-GST, N-Flag labeled tag.
- Application
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别名
CDK2; cyclin-dependent kinase 2; p33 protein kinase; cell devision kinase 2; cdc2-related protein kinase; cell division protein kinase 2; p33(CDK2)
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种属
Human
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表达系统
Baculovirus
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标签
N-His;N-GST;N-Flag
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P24941-1 (M1-L298 )&O96020-1
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表达区间
P24941-1 (M1-L298 )&O96020-1 (S2-H404 )
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CDK2 (Cyclin-Dependent Kinase 2) and CCNE2 (Cyclin E2) play crucial roles in the regulation of the cell cycle, particularly in the transition from the G1 phase to the S phase. Dysregulation of this pathway is often implicated in various cancers, as it can lead to uncontrolled cellular proliferation. The CDK2-CCNE2 complex is essential for the phosphorylation of target substrates, thereby promoting the progression of cells through the cycle. Research on CDK2-CCNE2 recombinant proteins has gained traction due to the potential for therapeutic interventions in cancer treatment. By understanding the structural and functional dynamics of this complex, scientists aim to identify specific inhibitors that can disrupt its activity in cancer cells. The study of CDK2-CCNE2 recombinant proteins provides insights into their interactions, regulatory mechanisms, and the downstream effects on cell cycle control, which are critical for developing targeted therapies. This research not only elucidates the molecular underpinnings of cell cycle regulation but also enhances the potential for devising novel drugs that can selectively target aberrant signaling pathways in cancer.
