Analytical Data
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Gene name
NEK3
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简介
NEK3 Protein influences neuronal morphogenesis and polarity through effects on microtubules. NEK3 Protein regulates microtubule acetylation in neurons. NEK3 Protein contributes to prolactin-mediated phosphorylation of PXN and VAV2. NEK3 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived NEK3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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Alternative Names
Serine/threonine-protein kinase Nek3; HSPK 36; NimA-related protein kinase 3
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Species
Mouse
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Source
Baculovirus
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Tag
N-His;N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
ABK42288
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Expression Region
M1-A509
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Protein Length
Full Length
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Molecular Weight
72 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
NEK3 (NIMA-related kinase 3) is a serine/threonine kinase that belongs to the NEK family, which is crucial for cell cycle regulation and mitotic progress. Recent studies have indicated that NEK3 plays a significant role in various cellular processes, including cilia formation, cell division, and response to DNA damage, making it a subject of growing interest in cancer and other diseases. The reorganization of NEK3 within tumor cells has been associated with altered cell proliferation and survival, highlighting its potential as a biomarker for cancer prognosis. Additionally, NEK3 has been implicated in the regulation of ciliary dynamics, suggesting its involvement in developmental disorders related to cilia dysfunction. Researchers are investigating the structural and functional characteristics of recombinant NEK3 protein to elucidate its exact role in signaling pathways and its interactions with other cellular components. By employing techniques such as protein purification and characterization, studies aim to develop NEK3-targeted therapies that could mitigate its aberrant activity in disease states, thereby advancing our understanding of its biological functions and therapeutic potential. Overall, the investigation of NEK3 recombinant protein presents a promising avenue for uncovering novel insights into its mechanisms and applications in health and disease.
