Analytical Data
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Gene name
TRIM16
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简介
TRIM16 protein is an essential E3 ubiquitin ligase that participates in autophagy reaction and ubiquitination during lysosome and phagosome damage. TRIM16 Protein, Human (sf9, His, Strep) is the recombinant human-derived TRIM16 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.
- Application
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Alternative Names
TRIM16; Tripartite motif-containing protein 16; E3 ubiquitin-protein ligase TRIM16; Estrogen-responsive B box protein
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Species
Human
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Source
Baculovirus
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Tag
N-StrepⅡ;N-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O95361
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Expression Region
A2-P564
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TRIM16, a member of the tripartite motif (TRIM) family of proteins, has garnered significant attention in recent years due to its roles in various biological processes, including immune response, cell proliferation, and apoptosis. The TRIM family is characterized by a RING finger domain, a B-box, and a coiled-coil region, which are crucial for its E3 ubiquitin ligase activity. Emerging evidence suggests that TRIM16 is involved in the regulation of inflammatory responses and may play a role in tumorigenesis. Studies have shown that TRIM16 can act as a tumor suppressor in certain cancers by modulating cellular pathways that control cell survival and proliferation. Additionally, TRIM16 has been implicated in the regulation of autophagy and the innate immune system, further highlighting its potential as a therapeutic target. Given its multifaceted functions, researchers are increasingly focusing on the mechanistic understanding of TRIM16’s interaction with cellular pathways, its post-translational modifications, and its impact on disease progression. Understanding these aspects could lead to new insights in developing novel therapeutic strategies for diseases where TRIM16 is dysregulated, such as cancer and autoimmune disorders. Therefore, ongoing studies involving TRIM16 recombinant proteins aim to elucidate its precise molecular mechanisms and physiological functions, paving the way for future clinical applications.
