Analytical Data
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Gene name
AURORA B
- Application
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Alternative Names
AURKB; AIK2; AIM1; ARK2; AurB; IPL1; AIM-1; STK12
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Species
Human
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Source
Baculovirus
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q96GD4-1
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Expression Region
M1-A344
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AA Sequence
MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVEHQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKSCTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEKVDLWCIGVLCYELLVGNPPFESASHNETYRRIVKVDLKFPASVPMGAQDLISKLLRHNPSERLPLAQVSAHPWVRANSRRVLPPSALQSVA
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Protein Length
Full Length
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Molecular Weight
68 kDa, based on SDS-PAGE under reducing conditions
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AURORA B, a serine/threonine kinase, is a crucial component of the chromosomal passenger complex (CPC) and plays a pivotal role in cell division, particularly during mitosis. Its main functions include regulating spindle assembly, chromosome alignment, and ensuring proper segregation during anaphase. Dysregulation of AURORA B expression or activity is linked to various cancers, making it a significant target for therapeutic interventions. Researchers have focused on characterizing the structure and function of AURORA B and its interactions with other cell cycle proteins to better understand its role in cell division. The study of AURORA B involves not only its catalytic function but also the dynamics of its localization and how it is regulated throughout the cell cycle. Recent advances in protein engineering and crystallography have facilitated the elucidation of its molecular structure, revealing insights into its activation mechanisms and how it interacts with inhibitors. Given its role in tumorigenesis, AURORA B is of great interest in the development of targeted cancer therapies, with inhibitors being explored in clinical trials. Understanding the biochemical pathways involving AURORA B is essential for developing novel strategies to combat cancer, highlighting the importance of ongoing research in this area.
