Analytical Data
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基因名
NlpD
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简介
NlpD, a vital bacterial protein, activates the cell wall hydrolase AmiC. Its crucial role in septal murein cleavage ensures proper daughter cell separation during division. NlpD's concerted action with AmiC maintains bacterial cell wall integrity, contributing to successful cell division progression. NlpD Protein, E.coli (His-SUMO) is the recombinant E. coli-derived NlpD protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Application
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别名
nlpD; b2742; JW2712Murein hydrolase activator NlpD
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种属
E.coli
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表达系统
E. coli
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标签
N-His;N-SUMO
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P0ADA3
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表达区间
C26-R379
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氨基酸序列
CSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR
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蛋白长度
Full Length of Mature Protein
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分子量
65 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
NlpD is a crucial protein in bacterial physiology and has garnered significant interest due to its role in bacterial cell envelope integrity and pathogenicity. It belongs to the group of non-classical proteins that are known to play essential roles in stress responses and virulence. Research has highlighted NlpD's involvement in the maintenance of the peptidoglycan layer, which is vital for bacterial shape and protection against environmental stresses. Understanding the structure and function of NlpD is important for elucidating the mechanisms of bacterial infection, as well as for potential therapeutic applications. Recent studies have focused on the recombinant expression of NlpD to facilitate functional assays and structural characterization. The production of recombinant NlpD allows for detailed studies of its biochemical properties, interactions with other cellular components, and its potential as a target for novel antimicrobial agents. As antibiotic resistance continues to rise, research into essential bacterial proteins like NlpD is critical for developing innovative strategies to combat infectious diseases. Overall, the study of NlpD and its recombinant forms represents a promising avenue for advancing our understanding of bacterial physiology and improving public health outcomes.
