Analytical Data
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Gene name
DEFA1
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简介
The DEFA1 protein is a multifunctional effector in innate immunity that exhibits antibiotic-like properties against bacteria, fungi, and viruses. It promotes immune defense by activating antigen-presenting cells (APCs) and inhibiting bacterial cell wall synthesis by interacting with lipid II. DEFA1 Protein, Human (HEK293, Twin Strep) is the recombinant human-derived DEFA1 protein, expressed by HEK293, with C-Twin-Strep labeled tag.
- Application
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Species
Human
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Source
HEK293
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Tag
C-Twin-Strep
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P59665
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Expression Region
D39-C94
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Molecular Weight
11-14 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Defensins are a class of small, cationic peptides that play critical roles in the innate immune response, and among them, DEFA1 is a member of the human alpha-defensin family. DEFA1 is primarily expressed in neutrophils and is known for its antimicrobial properties, particularly against bacteria and fungi. Research on DEFA1 is particularly relevant due to its potential implications in understanding host defense mechanisms, inflammatory responses, and the development of novel therapeutic agents. The study of DEFA1 recombining proteins involves expressing the gene in laboratory settings to produce the protein in sufficient quantities for detailed analysis. This research aims to elucidate the structural and functional characteristics of DEFA1, allowing for better insights into its antimicrobial mechanisms and interactions with pathogens. Additionally, the exploration of DEFA1 variants can provide information on genetic factors that influence susceptibility to infections and diseases. As antibiotic resistance continues to pose a significant public health challenge, DEFA1 and other defensins are being investigated for their potential as alternative antimicrobial agents. The ongoing research in the field of DEFA1 recombinant proteins underscores its significance in linking basic immunology with translational medicine, aiming to harness the natural defense mechanisms for therapeutic applications.
